Uridine phosphorylase from Schistosoma mansoni.

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Purine nucleoside phosphorylase from Schistosoma mansoni in complex with ribose-1-phosphate

Schistosomes are blood flukes which cause schistosomiasis, a disease affecting approximately 200 million people worldwide. Along with several other important human parasites including trypanosomes and Plasmodium, schistosomes lack the de novo pathway for purine synthesis and depend exclusively on the salvage pathway for their purine requirements, making the latter an attractive target for drug ...

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Two distinct pyrimidine nucleosidases, isolated from Escheri&a coli, show complete specificity for the pentose moiety of the nucleoside. Pyrimidine deoxyribonucleoside phosphorylase (1) shows complete specificity for the deoxyribosyl group, and uridine phosphorylase (2) for the ribosyl group. Mammalian uridine phosphorylase preparations (3, 4), however, have appreciable deoxyuridine phosphoryla...

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Crystal Structure of Schistosoma mansoni Adenosine Phosphorylase/5’-Methylthioadenosine Phosphorylase and Its Importance on Adenosine Salvage Pathway

Schistosoma mansoni do not have de novo purine pathways and rely on purine salvage for their purine supply. It has been demonstrated that, unlike humans, the S. mansoni is able to produce adenine directly from adenosine, although the enzyme responsible for this activity was unknown. In the present work we show that S. mansoni 5´-deoxy-5´-methylthioadenosine phosphorylase (MTAP, E.C. 2.4.2.28) i...

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a Departamento de Bioquı́mica Médica, Uni6ersidade Federal do Rio de Janeiro, Cidade Uni6ersitária, 21941-590, Rio de Janeiro, RJ, Brazil b Di6isão de Quı́mica, Setor de Quı́mica Orgânica, CENPES, Petrobrás, Cidade Uni6ersitária, 21949-900, Rio de Janeiro, RJ, Brazil c Centro de Biociências e Biotecnologia, Uni6ersidade Estadual do Norte Fluminense, Campos dos Goytacazes, RJ, 28015-620, Brazil d I...

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(1)Institute of Tropical Medicine, São Paulo, SP, Brasil. (2)Division of Nephrology, “Hospital das Clinicas”, University of S. Paulo, Medical School, S. Paulo, SP, Brasil. (3)São Paulo Federal University Medical School, Dept. of Microbiology, Immunology, and Parasitology, S. Paulo, SP, Brazil. Correspondence to: Dr. Thales De Brito, Av. Dr. Enéas de Carvalho Aguiar 470, 05403–000 S. Paulo, SP, ...

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ژورنال

عنوان ژورنال: Journal of Biological Chemistry

سال: 1988

ISSN: 0021-9258

DOI: 10.1016/s0021-9258(18)68752-6